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marathon readytm mouse brain cdna library  (TaKaRa)


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    TaKaRa marathon readytm mouse brain cdna library
    Expression analysis of m-Golsyn transcripts in mouse tissues. <t>cDNA</t> fragments of m-Golsyn type 1 and type 2 were amplified by using the primer sets described under Materials and Methods. (A) The positions of the two sets of primers are indicated by the arrows. (B) The cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using first-strand cDNAs derived from nine different mouse tissues. Lane 1 in (B) is a negative control lacking template cDNA. PCR was performed with cDNA derived from 0.15 μg of RNA for the amplification of m-Golsyn cDNAs and mouse actin cDNA.
    Marathon Readytm Mouse Brain Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/marathon+readytm+cdna+library/Advantage+2+Polymerase+Mix/pmc06032447-40-10-21
    Average 96 stars, based on 3309 article reviews
    marathon readytm mouse brain cdna library - by Bioz Stars, 2026-09
    96/100 stars

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    1) Product Images from "Molecular Cloning of the m- Golsyn Gene and its Expression in the Mouse Brain"

    Article Title: Molecular Cloning of the m- Golsyn Gene and its Expression in the Mouse Brain

    Journal: Gene Expression

    doi:

    Expression analysis of m-Golsyn transcripts in mouse tissues. cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using the primer sets described under Materials and Methods. (A) The positions of the two sets of primers are indicated by the arrows. (B) The cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using first-strand cDNAs derived from nine different mouse tissues. Lane 1 in (B) is a negative control lacking template cDNA. PCR was performed with cDNA derived from 0.15 μg of RNA for the amplification of m-Golsyn cDNAs and mouse actin cDNA.
    Figure Legend Snippet: Expression analysis of m-Golsyn transcripts in mouse tissues. cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using the primer sets described under Materials and Methods. (A) The positions of the two sets of primers are indicated by the arrows. (B) The cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using first-strand cDNAs derived from nine different mouse tissues. Lane 1 in (B) is a negative control lacking template cDNA. PCR was performed with cDNA derived from 0.15 μg of RNA for the amplification of m-Golsyn cDNAs and mouse actin cDNA.

    Techniques Used: Expressing, Amplification, Derivative Assay, Negative Control

    Related Articles

    Amplification:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. The full-length cDNA of mouse LC3B (GeneBank accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_026160","term_id":"158508694","term_text":"NM_026160"}} NM_026160 ) was amplified by PCR from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc.) using the appropriate primers (forward primer: 5′-ATGCCGTCCGAGAAGACCTTCAAGCAG-3′, reverse primer: 5′-TTACACAGCCATTGCTGTCCCGAATGT-3′) and cloned into pLexA and pB42AD (Clontech Laboratories, Inc.). ..

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. Plasmid constructs The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: The proteins were processed for 10% SDS-PAGE and immunoblot analysis with antibodies against KLC1 (Abcam), KIF5B ( 6 ), LC3B (1:1,000, cat. no. ab48394; Abcam) and FLAG (1:2,000, cat. no. F7425; Sigma-Aldrich). .. The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor
    Article Snippet: SuperdexTM Peptide HR10/30 and SuperdexTM 200 10/300 GL columns were obtained from Amersham Biosciences. .. The cDNA fragment of a truncated form of CSS3 (ChSy-2), lacking the first 129 N-terminal amino acids of CSS3, was amplified by RT (reverse transcriptase)-PCR with the Marathon-ReadyTM cDNA library derived from human brain (Clontech) as a template using a 5′-primer (5′-CCCTCGAGGGCCGAGGGGGAGCCCGA-3′) containing an in-frame XhoI site and a 3′-primer (5′-CCCTCGAGCTGTCAGGAGAGAGTTCGATT-3′) containing a XhoI site located 3 bp downstream of the stop codon. .. The PCR was carried out using KOD -Plus DNA polymerase (Toyobo Biochemicals) for 30 cycles of 94 °C for 30 s, 58 °C for 30 s and 68 °C for 150 s in 5% (v/v) DMSO.

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    Polymerase Chain Reaction:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. The full-length cDNA of mouse LC3B (GeneBank accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_026160","term_id":"158508694","term_text":"NM_026160"}} NM_026160 ) was amplified by PCR from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc.) using the appropriate primers (forward primer: 5′-ATGCCGTCCGAGAAGACCTTCAAGCAG-3′, reverse primer: 5′-TTACACAGCCATTGCTGTCCCGAATGT-3′) and cloned into pLexA and pB42AD (Clontech Laboratories, Inc.). ..

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. Plasmid constructs The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: The proteins were processed for 10% SDS-PAGE and immunoblot analysis with antibodies against KLC1 (Abcam), KIF5B ( 6 ), LC3B (1:1,000, cat. no. ab48394; Abcam) and FLAG (1:2,000, cat. no. F7425; Sigma-Aldrich). .. The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    cDNA Library Assay:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. The full-length cDNA of mouse LC3B (GeneBank accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_026160","term_id":"158508694","term_text":"NM_026160"}} NM_026160 ) was amplified by PCR from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc.) using the appropriate primers (forward primer: 5′-ATGCCGTCCGAGAAGACCTTCAAGCAG-3′, reverse primer: 5′-TTACACAGCCATTGCTGTCCCGAATGT-3′) and cloned into pLexA and pB42AD (Clontech Laboratories, Inc.). ..

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. Plasmid constructs The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: The proteins were processed for 10% SDS-PAGE and immunoblot analysis with antibodies against KLC1 (Abcam), KIF5B ( 6 ), LC3B (1:1,000, cat. no. ab48394; Abcam) and FLAG (1:2,000, cat. no. F7425; Sigma-Aldrich). .. The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor
    Article Snippet: SuperdexTM Peptide HR10/30 and SuperdexTM 200 10/300 GL columns were obtained from Amersham Biosciences. .. The cDNA fragment of a truncated form of CSS3 (ChSy-2), lacking the first 129 N-terminal amino acids of CSS3, was amplified by RT (reverse transcriptase)-PCR with the Marathon-ReadyTM cDNA library derived from human brain (Clontech) as a template using a 5′-primer (5′-CCCTCGAGGGCCGAGGGGGAGCCCGA-3′) containing an in-frame XhoI site and a 3′-primer (5′-CCCTCGAGCTGTCAGGAGAGAGTTCGATT-3′) containing a XhoI site located 3 bp downstream of the stop codon. .. The PCR was carried out using KOD -Plus DNA polymerase (Toyobo Biochemicals) for 30 cycles of 94 °C for 30 s, 58 °C for 30 s and 68 °C for 150 s in 5% (v/v) DMSO.

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    Clone Assay:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. The full-length cDNA of mouse LC3B (GeneBank accession number: {"type":"entrez-nucleotide","attrs":{"text":"NM_026160","term_id":"158508694","term_text":"NM_026160"}} NM_026160 ) was amplified by PCR from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc.) using the appropriate primers (forward primer: 5′-ATGCCGTCCGAGAAGACCTTCAAGCAG-3′, reverse primer: 5′-TTACACAGCCATTGCTGTCCCGAATGT-3′) and cloned into pLexA and pB42AD (Clontech Laboratories, Inc.). ..

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    Plasmid Preparation:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. Plasmid constructs The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    Construct:

    Article Title: Interaction of FUN14 domain containing 1, a mitochondrial outer membrane protein, with kinesin light chain 1 via the tetratricopeptide repeat domain
    Article Snippet: .. Plasmid constructs The mouse KLC1 cDNA fragment corresponding to the TPR domain (amino acids 80–541) was amplified by polymerase chain reaction (PCR) from the Marathon-ReadyTM cDNA library (Clontech Laboratories, Inc., Palo Alto, CA, USA) using the appropriate primers (forward primer: 5′-AGCGAGGCGCAGGTGATGATGGCG-3′, reverse primer: 5′-AGTGCCATCCCCATTCCACTCTAC-3′). .. The PCR reaction was performed in a total volume of 25 μl containing ~50 ng cDNA library, 200 mM deoxynucleotide triphosphates (dNTPs), 1X reaction buffer (MBI Fermentas, St. Leon-Rot, Germany), 1.5 mM MgCl 2 , and 2-unit Taq polymerase (MBI Fermentas).

    Article Title: APP Tail 1 (PAT1) Interacts with Kinesin Light Chains (KLCs) through the Tetratricopeptide Repeat (TPR) Domain
    Article Snippet: .. Plasmid constructs Full-length mouse KLC1 (accession NM_001081959), KLC2 (accession NM_008451), and PAT1 (accession NM_025825) were amplified by polymerase chain reaction (PCR) from Marathon-ReadyTM cDNA library (Clontech, Palo Alto, CA, USA) and cloned into pGEM T-easy vector (Promega Corp, Madison, WI, USA). ..

    Derivative Assay:

    Article Title: Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor
    Article Snippet: SuperdexTM Peptide HR10/30 and SuperdexTM 200 10/300 GL columns were obtained from Amersham Biosciences. .. The cDNA fragment of a truncated form of CSS3 (ChSy-2), lacking the first 129 N-terminal amino acids of CSS3, was amplified by RT (reverse transcriptase)-PCR with the Marathon-ReadyTM cDNA library derived from human brain (Clontech) as a template using a 5′-primer (5′-CCCTCGAGGGCCGAGGGGGAGCCCGA-3′) containing an in-frame XhoI site and a 3′-primer (5′-CCCTCGAGCTGTCAGGAGAGAGTTCGATT-3′) containing a XhoI site located 3 bp downstream of the stop codon. .. The PCR was carried out using KOD -Plus DNA polymerase (Toyobo Biochemicals) for 30 cycles of 94 °C for 30 s, 58 °C for 30 s and 68 °C for 150 s in 5% (v/v) DMSO.



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    Image Search Results


    Expression analysis of m-Golsyn transcripts in mouse tissues. cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using the primer sets described under Materials and Methods. (A) The positions of the two sets of primers are indicated by the arrows. (B) The cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using first-strand cDNAs derived from nine different mouse tissues. Lane 1 in (B) is a negative control lacking template cDNA. PCR was performed with cDNA derived from 0.15 μg of RNA for the amplification of m-Golsyn cDNAs and mouse actin cDNA.

    Journal: Gene Expression

    Article Title: Molecular Cloning of the m- Golsyn Gene and its Expression in the Mouse Brain

    doi:

    Figure Lengend Snippet: Expression analysis of m-Golsyn transcripts in mouse tissues. cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using the primer sets described under Materials and Methods. (A) The positions of the two sets of primers are indicated by the arrows. (B) The cDNA fragments of m-Golsyn type 1 and type 2 were amplified by using first-strand cDNAs derived from nine different mouse tissues. Lane 1 in (B) is a negative control lacking template cDNA. PCR was performed with cDNA derived from 0.15 μg of RNA for the amplification of m-Golsyn cDNAs and mouse actin cDNA.

    Article Snippet: Reagents The following materials were purchased from the sources indicated: Marathon-ReadyTM mouse brain cDNA library and Advantage 2 polymerase mix from Clontech Co. Ltd. (Palo Alto, CA); TA Cloning kits from Invitrogen Co. (Carlsbad, CA); Histofine from Nichirei Co. (Tokyo, Japan); Vectastain ABC kit from Vector Laboratories, Inc. (Burlingame, CA).

    Techniques: Expressing, Amplification, Derivative Assay, Negative Control